UVP DigiDoc-It Bedienungsanleitung Seite 60
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CHAPTER FIVE: ACQUIRING AND MANAGING IMAGES
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Current Black Level: Stacking frames also stacks noise along with it. To clip noise from
each image before stacking, it is important to set the black-level. Value of the darkest
pixel in the image (which will typically be noise) should be set inside the spin-box. Pixel
values less than this value are not recorded or clipped during acquisition and before
stacking.
BIOCHEMI HR/CHEMI HR 410
This section pertains to several UVP cameras:
BioChemi HR Camera
Chemi HR 410 Camera
To view the plug-in for any of the above cameras, select View > Plugins > BioChemi HR/Chemi
410 Camera Plug-in.
**
- Life Science Software 1
- TABLE OF CONTENTS 2
- END USER LICENSE AGREEMENT 7
- VISIONWORKS®LS 7
- DOC-IT®LS 9
- SOFTWARE 11
- INSTALLATION INSTRUCTIONS 12
- SECURE USER ACCOUNTS 13
- USERNAMES AND PASSWORDS 14
- To Edit a new user: 15
- MATRIX OF USER RIGHTS 17
- ACTIVATION 18
- HOW TO REGISTER THE SOFTWARE 23
- MAIN WINDOW 24
- Area Density Available 27
- TOOLBARS 29
- PLUG-IN MODULES 31
- Rearranging Plug-in Modules 32
- IMAGE WINDOWS 33
- Organizing Image Windows 34
- STATUS BAR 35
- HAPTER FOUR: PLUG-IN MODULES 36
- DIGITAL VIDEO PLAYER (DVP) 37
- 3 DIMENSIONAL SURFACE PLOTS 37
- IMAGE FILTERS 39
- OLYMPUS CAMERA (DIGI CAMERA) 39
- ZOOM/PAN 40
- IMAGE CONTROLS 42
- PSEUDOCOLOR 43
- To Apply a Pseudocolor 44
- HISTOGRAM CONTROLS 45
- AREA DENSITY 46
- CANON CAMERA 46
- 1D ANALYSIS 47
- HAMAMATSU CAMERA 48
- COHU CAMERA 48
- COLONY COUNTING 49
- FTP TRANSFER 50
- DARKROOM AND LENS 51
- DARKROOM CONNECTIVITY 52
- SELECTING A CAMERA 54
- OPTICHEMI 55
- Hamamatsu Camera Plug-In 56
- Hamamatsu Capture Templates 57
- Binning Modes 58
- Exposure Time Integration 58
- BIOCHEMI HR/CHEMI HR 410 60
- FLUOR / CHEMI CAMERAS 62
- Cohu Camera Plugin 63
- Cohu Capture Template 63
- DIGI CAMERA 66
- Canon Capture Plug-in 67
- To Unlock the Focus Lock 68
- Canon Capture Templates 69
- SCANNING IMAGES 70
- HOW TO ACCESS THE PLAYER 72
- PLAYER FEATURES 72
- PLAYER OPTIONS 73
- .AVI FILES 76
- TRANSFERRING IMAGES 77
- IMAGE FILES 78
- The Extended Attributes File 79
- To Transfer an Image 80
- CHAPTER EIGHT: IMAGE EDITING 81
- USING SELECTION TOOLS 82
- To Select a Region 83
- To Adjust the Selection 83
- To Cancel the Selection 83
- PASTE SPECIAL 84
- To Merge Two Images 85
- HAPTER EIGHT: IMAGE EDITING 86
- CHAPTER NINE: IMAGE FILTERS 87
- HAPTER NINE: IMAGE FILTERS 88
- FLIP HORIZONTALLY 90
- FLIP VERTICALLY 90
- RESIZE 90
- REDUCE TO MONO 91
- CONVERT 91
- REMOVE NOISE 92
- DESPECKLE 92
- SHARPEN 93
- STARFIELD SUBTRACTION 94
- DUPLICATING IMAGES 95
- BURN CHANGES ON NEW IMAGES 95
- BACKGROUND SUBTRACTION 95
- CHAPTER TEN: ANNOTATIONS 96
- TYPES OF ANNOTATION 97
- 4. Click OK 99
- CREATING ANNOTATIONS 100
- THE TEXT ANNOTATION WINDOW 101
- SELECTING ANNOTATIONS 102
- FORMATTING ANNOTATIONS 103
- DELETING ANNOTATIONS 104
- OVERVIEW 105
- CALIBRATING IMAGE SCALE 106
- IMAGE HISTORY 106
- UNDERSTAND A PIXEL 107
- The Meaning of Image Depth 108
- Displaying Depth 108
- REPORTS 109
- To Print an Image 110
- NAVIGATION 111
- 1D Analysis Plugin Buttons 112
- 1D Analysis Settings 114
- To Perform a Basic Search 115
- To Adjust Search Parameters 115
- MODIFYING BANDS 117
- Deleting Bands 118
- CALIBRATION 120
- Adding Rf Lines Manually 121
- MOLECULAR WEIGHT CALIBRATION 122
- Calibrating a Lane 124
- Using the Stretch Factor 124
- Exact Placement of Bands 125
- BACKGROUND CORRECTION 126
- No Background Correction 127
- Straight Line 127
- Joined Valleys 127
- CONCENTRATION CALIBRATION 128
- Selecting Unit Type 129
- SELECTING DATA POINTS 130
- Selecting Data Points 131
- Editing Data Points 131
- Deleting Data Points 131
- SELECTING CURVE TYPE 132
- LANE INFORMATION 134
- BAND INFORMATION 135
- LANE PROFILE GRAPH 136
- Displaying Multiple Lanes 137
- To Change the Selected Lane: 137
- Axis Options 137
- Display Options 138
- Color Options 138
- DATA EXPLORER 139
- Filtering Data 140
- To Select Filter Data Fields 141
- To Show Filtered Data 141
- Export Data Explorer Reports 142
- Fixed Reports 143
- Analysis Settings 143
- Analysis Lanes 144
- Analysis Bands 145
- Lane Profile Report 145
- SEVENTEEN: COLONY COUNTING 146
- MANUAL COLONY COUNTING 147
- Apply filters – Step 4 of 5 150
- Finish – Step 5 of 5 151
- The result window 152
- Colony Counting Menu Bar 154
- PURPOSE 157
- AREA DENSITY OPTIONS 157
- STEPS TO FOLLOW 160
- Define Background 162
- Update the Results 163
- INTENSITY CALIBRATION CURVES 164
- Optical Density 165
- Grey Levels 165
- To Calibrate the Intensity 166
- Calibration Always Positive 167
- Monotonic Calibration 168
- SPATIAL CALIBRATION 169
- Compliance 170
- Party Software Agreements 172
- 2: GLOSSARY 178
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