Uvp Doc-It Life Science Bedienungsanleitung Seite 83
- Seite / 111
- Inhaltsverzeichnis
- LESEZEICHEN
Bewertet. / 5. Basierend auf Kundenbewertungen
Performing 1D Analysis
78
Background correction is necessary to account for possible variable illumination or overexposure during
image capturing, LS software offers options to apply mathematical background correction. These options
generally remove background "noise" and elevated levels of pixel intensity due to excess exposure,
highlighting data. There are four corrections options that are explained in the Specific Correction Options
section of this chapter.
From the Analysis > 1D Analysis > Master Tools > Background Correction or the 1D Analysis plugin
dropdown menu a new list appears.
Using the 1D Analysis Plugin
From the list select which type of background correction, if any, that the software should perform.
Viewing Background Correction in the Lane Profile Graph
Choose the Analysis > 1D Analysis > Master Tools > Lane Profile Graph or click on Lane profile
graph from the 1D Analysis Plugin.
On the graph, in the second box below Background Correction, click the down arrow to show the drop-
down menu of the background correction options. Click the appropriate correction type. The graph will
now display the background correction in the selected lane as a dotted line.
Specific Correction Options
LS Software offers background correction options.
No background correction
Straight line
Joined valleys
Area between the lines
Rolling disc
No Background Correction
Selecting this option on the menu leaves the image uncorrected for overexposure, "as is."
Straight Line
Selecting this option tells LS Software to place a straight (but not necessarily horizontal) line under the
lowest points at the beginning and end of each lane. LS removes the area of the graph under the straight
line, so that all remaining values are emphasized. Straight line correction tends to correct well for
overexposure, and for variable illumination that is focused on an edge or corner of an image.
Note:
Straighten lines by using right-clicking the mouse button to open the
shortcut menu.
Joined Valleys
Selecting this option accentuates the data by telling LS software to join lines between the lowest point, or
"valley", before the first band, between each pair of bands, and after the last band. Intensities above the
valleys (band data) are emphasized. Joined valleys can perform well in a variable illumination condition
where the "bright spot" is somewhere in the middle of the image, and where bands are sharply defined
- Web site: uvp.com 1
- Table of Contents 2
- Introduction 6
- Minimum System Requirements 7
- On the Fly Activation 8
- User Administration 9
- Single-User License 9
- Usernames and Passwords 10
- Technical Support 12
- License Agreement 13
- Limitation of Liability 14
- Navigating the Software 15
- Toolbars 16
- Image Windows 18
- Status Bar 19
- Using Plug-In Modules 20
- Positioning Plug-in Modules 21
- Histogram Controls 22
- Digital Video Player 22
- Using the Player 23
- Player Features 23
- Player Options 24
- Image Controls Plug-In 26
- Change Contrast 27
- Change Gamma 27
- 3D Plots 28
- FTP Transfer 29
- Log Viewer 29
- Zoom/Pan 29
- Using Zoom In or Out 30
- Magnify Part of an Image 30
- Camera Plug-In 32
- Capturing Images 35
- Creating Templates 36
- Creating Colony Templates 37
- Editing Images 39
- Undo and Redo 40
- Using Image Filters 42
- Background Filter 43
- Burn Changes to a New Image 44
- Convert Image 44
- Duplicating Images 44
- Flip Image 44
- Image Enhancement Filters 45
- Image History 46
- Measurement Tools 47
- Merging Two Images 48
- Obtaining Image Information 49
- Pseudocolor 50
- Reduce Image to Mono 51
- Resize Image 51
- Rotate and Align Image 52
- Rulers 53
- Scanning Images 53
- Spatial Calibration 54
- Annotations 56
- New Annotation Window 57
- Selecting Annotations 58
- Formatting Annotations 60
- Deleting Annotations 60
- Creating Annotations 61
- Loading and Saving Images 62
- Image File Types 63
- The Extended Attributes File 64
- Performing 1D Analysis 65
- Finding Lanes and Bands 67
- 1D Analysis Settings 70
- Resize Band Extents 71
- Delete Lanes and Bands 72
- Lane and Band Information 73
- Lane Profile Graph 74
- Display Multiple Lanes 75
- Change the Selected Lane 75
- Axis Options 75
- Display Options 75
- Selecting Background Color 76
- Overview 77
- Filtering Data 77
- Export Data Explorer Reports 80
- Using the 1D Analysis Plugin 83
- Specific Correction Options 83
- Concentration Calibration 84
- Unit Type 85
- Molecular Weight Calibration 88
- Using the Stretch Factor 89
- Exact Placement of Bands 90
- Adding Rf Lines Manually 91
- Generating Dendrogram Graphs 93
- Modify Dendrogram Graphs 93
- Creating clusters 94
- Linkage rules 94
- Performing Colony Counting 96
- Label Type 97
- Colony Marking 97
- Label Color 97
- Automatic Counting 98
- Manual Counting 98
- Edit Colonies 100
- Add Colonies 100
- Delete Colonies 101
- Split Colonies 101
- Merging Colonies 102
- Spiral Counting 102
- Zone Analysis 103
- Reporting Functions 104
- Classes 104
- Statistics 105
- Distribution 105
- Printing Reports and Images 106
- Purpose 108
- Features 108
- Glossary 110
Verwandte Produkte und Handbücher für Software Uvp Doc-It Life Science
Software Uvp Life Science Bedienungsanleitung
(269 Seiten)
(269 Seiten)
Casio XJ-460, XJ-560 manuels
Manuels d'utilisation et guides de l'utilisateur pour Non Casio XJ-460, XJ-560.
Nous fournissons des manuels en pdf 5 Casio XJ-460, XJ-560 à télécharger gratuitement par type de document : Manuel de l'utilisateur
Kommentare zu diesen Handbüchern