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LS Software User Guide

136

Similarity and distance between lanes

Based on band matching, the similarity between two lanes L1 and L2 can be evaluated. Notations:

• B1 is the number of bands in lane L1

• B2 is the number of bands in lane L2

• M is the number of matching bands in each lane, therefore

The similarity between two lanes can be measured using Dice or Jaccard scores. Dice similarity

formula is:

Jaccard similarity formula is:

The opposite to the concept of similarity is the concept of distance:

Distance values will be used to create the dendrogram.

Create Clusters

Initially, each lane has its own cluster. Then, repeatedly, a linkage rule (see below) is used to merge

smaller groups into larger clusters, until all the clusters have been combined into a single cluster. The

result is a hierarchy of clusters. Moving up the hierarchy contains clusters with more but less similar

lanes. Lanes that are very similar to each other will appear together in clusters near the bottom of

hierarchy.

The dendrogram shows the links that have been made between the clusters to form larger clusters

&endash; the shorter the distance between items in the dendrogram, the more similar they are.

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Uvp Life Science Bedienungsanleitung Seite 150